Review: Practical Design and Analysis of 2-Colour cDNA Microarray Experiments

This review paper, is aimed at biological researchers who are interested in or have begun to use cDNA microarrays for their investigations. Large microarray studies typically involve a multidisciplinary team with various groups performing different aspects of the same experiment. This approach means that microarrays are less accessible to new researchers than more traditional biological techniques. This review aims to make current techniques of statistical design, normalisation and linear analysis of cDNA microarray experiments accessible to a wider community. These methods will be illustrated with examples that use freely-available packages implemented in Bioconductor and R.